| # | LOT_ID | Target | Assay_type | Assay_name | Value_type | Value | Unit | Relation | Value_comments | Conc_uM | Cell_line | Organism | Reference | Data_source | DB_ID | Memo |
|---|
| 1 | HG-0260086-0-00 |
Cav1.2 |
binding |
Displacement of [3H]nitrendipine from dihydropyridine receptor of guinea pig myocardial membranes |
Kd |
0.4 |
nM |
= |
|
|
|
Cavia porcellus |
J. Med. Chem., 1995, 38 (1), 119-129 |
ChEMBL |
1086672 |
data from ChEMBL25 |
| 2 | HG-0260086-0-00 |
Cav1.2 |
binding |
Inhibition of [3H]nitrendipine binding to L-type calcium channel dihydropyridine site of porcine cardiac sarcolemma membrane vesicles |
Ki |
1 |
nM |
= |
|
|
|
Sus scrofa |
J. Med. Chem., 1987, 30 (4), 690-695 |
ChEMBL |
106372 |
data from ChEMBL25 |
| 3 | HG-0260086-0-00 |
Cav1.2 |
binding |
Bmax1 from dihydropyridine receptor binding assay in guinea pig myocardial membranes |
Bmax1 |
100 |
% |
= |
|
|
|
Cavia porcellus |
J. Med. Chem., 1988, 31 (5), 936-944 |
ChEMBL |
976057 |
data from ChEMBL25 |
| 4 | HG-0260086-0-00 |
Cav1.2 |
binding |
Dissociation constant for inhibition of [3H]nitrendipine binding to guinea pig myocardial membranes |
Kd1 |
0.39 |
nM |
= |
|
|
|
Cavia porcellus |
J. Med. Chem., 1988, 31 (5), 936-944 |
ChEMBL |
976058 |
data from ChEMBL25 |
| 5 | HG-0260086-0-00 |
Cav1.2 |
patch clamp |
Inhibition of L-type calcium channel measured using 2-electrode voltage-clamp in Chinese hamster ovary cells heterologically expressing alpha-1C subunit |
IC50 |
50 |
nM |
= |
|
|
CHO |
Cricetulus griseus |
Journal of Applied Toxicology, 2012, 32 (), 858-866 |
ChEMBL |
15373257 |
data from ChEMBL25 |
| 6 | HG-0260086-0-00 |
Cav1.2 |
patch clamp |
Inhibition of L-type calcium channel measured using 2-electrode voltage-clamp in human embryonic kidney cells heterologically expressing alpha-1C subunit |
IC50 |
55 |
nM |
= |
|
|
HEK293 |
Homo sapiens |
Journal of Applied Toxicology, 2012, 32 (), 858-866 |
ChEMBL |
15373260 |
data from ChEMBL25 |
| 7 | HG-0260086-0-00 |
Cav1.2 |
patch clamp |
Inhibition of L-type calcium channel measured using 2-electrode voltage-clamp in human embryonic kidney cells heterologically expressing alpha-1C subunit |
IC50 |
22 |
nM |
= |
|
|
HEK293 |
Homo sapiens |
Journal of Applied Toxicology, 2012, 32 (), 858-866 |
ChEMBL |
15373259 |
data from ChEMBL25 |
| 8 | HG-0260086-0-00 |
Cav1.3 |
other |
Inhibition of human Cav1.3 channel in human SH-SY5Y cells assessed as 70 mM K+ induced calcium elevation compound treated 15 mins before stimulus by Fluo-4/AM assay |
IC50 |
1350 |
nM |
= |
|
|
SH-SY5Y |
Homo sapiens |
J. Med. Chem., 2014, 57 (10), 4313-4323 |
ChEMBL |
14664834 |
data from ChEMBL25, unknown assay type |
| 9 | HG-0260086-0-00 |
Sodium channel alpha subunit |
other |
Inhibition of Na channel (species unknown) |
IC50 |
37000 |
nM |
= |
|
|
|
Homo sapiens |
Cardiovasc. Res., 2011, 91 (), 53-61 |
ChEMBL |
15257905 |
data from ChEMBL25, unknown assay type |
| 10 | HG-0260086-0-00 |
Voltage-gated L-type calcium channel |
other |
Calcium channel antagonistic activity relative to 2,6-Dimethyl-4-(2-nitro-phenyl)-1,4-dihydro-pyridine-3,5-dicarboxylic acid dimethyl ester(=100) |
Relative activity |
100 |
|
= |
|
|
|
Cavia porcellus |
J. Med. Chem., 1982, 25 (2), 126-131 |
ChEMBL |
647066 |
data from ChEMBL25, unknown assay type |
| 11 | HG-0260086-0-00 |
Voltage-gated L-type calcium channel |
binding |
Free energy of binding (delta G) for inactivated state (is) of voltage-gated L-type [Ca2+] channel(VGCCs) |
Binding energy |
-13.18 |
kCal M-1 |
= |
|
|
|
Homo sapiens |
J. Med. Chem., 1999, 42 (12), 2204-2211 |
ChEMBL |
586704 |
data from ChEMBL25 |
| 12 | HG-0260086-0-00 |
Voltage-gated L-type calcium channel |
binding |
Free energies of binding (delta G) in the Resting state (rs) of voltage-gated L-type [Ca2+] channel(VGCCs) |
Binding energy |
-10.5 |
kCal M-1 |
= |
|
|
|
Homo sapiens |
J. Med. Chem., 1999, 42 (12), 2204-2211 |
ChEMBL |
586703 |
data from ChEMBL25 |
| 13 | HG-0260086-0-00 |
Voltage-gated L-type calcium channel |
binding |
Evaluated for the free energies of binding (delta G) (in the resting state (rs) of voltage-gated L-type [Ca2+] channel (VGCCs)) |
Binding energy |
-12.38 |
kCal M-1 |
= |
|
|
|
Homo sapiens |
J. Med. Chem., 1999, 42 (12), 2204-2211 |
ChEMBL |
585491 |
data from ChEMBL25 |
| 14 | HG-0260086-0-00 |
Voltage-gated L-type calcium channel |
binding |
Negative log antagonist concentration causing 50% displacement of [3H]nifedipine from guinea pig ileal smooth muscle membrane. |
-Log KD50 |
9.08 |
|
= |
|
|
|
Cavia porcellus |
J. Med. Chem., 1987, 30 (4), 640-646 |
ChEMBL |
1190527 |
data from ChEMBL25 |
| 15 | HG-0260086-0-00 |
Voltage-gated L-type calcium channel |
other |
Inhibition of voltage-gated L-type Ca channel (species unknown) |
IC50 |
60 |
nM |
= |
|
|
|
Homo sapiens |
Cardiovasc. Res., 2011, 91 (), 53-61 |
ChEMBL |
15257934 |
data from ChEMBL25, unknown assay type |
| 16 | HG-0260086-0-00 |
Voltage-gated L-type calcium channel |
patch clamp |
Inhibition of Cav1.2 current measured using QPatch automatic path clamp system in CHO cells expressing Cav1.2, beta-2 and alpha-2/delta-1 subunits |
IC50 |
10 |
nM |
= |
|
|
CHO |
Cricetulus griseus |
Scientific Reports, 2013, 3 (), 1-7 |
ChEMBL |
15373341 |
data from ChEMBL25 |
| 17 | HG-0260086-0-00 |
Kv1.5 |
patch clamp |
Inhibition of human Kv1.5 channel expressed in mouse L929 cells by EP voltage clamp technique |
IC50 |
6100 |
nM |
= |
|
|
L929 |
Homo sapiens |
Bioorg. Med. Chem. Lett., 2008, 18 (24), 6381-6385 |
ChEMBL |
2589395 |
data from ChEMBL25 |
| 18 | HG-0260086-0-00 |
hERG |
other |
Inhibition of human Potassium channel HERG expressed in mammalian cells |
IC50 |
50118.7 |
nM |
= |
|
|
|
Homo sapiens |
Bioorg. Med. Chem. Lett., 2003, 13 (16), 2773-2775 |
ChEMBL |
1028643 |
data from ChEMBL25 Potential duplicate., unknown assay type |
| 19 | HG-0260086-0-00 |
hERG |
other |
Inhibitory concentration against potassium channel HERG |
IC50 |
50118.7 |
nM |
= |
|
|
|
Homo sapiens |
Bioorg. Med. Chem. Lett., 2005, 15 (11), 2886-2890 |
ChEMBL |
1523841 |
data from ChEMBL25 Potential duplicate., unknown assay type |
| 20 | HG-0260086-0-00 |
hERG |
other |
Inhibitory concentration against IKr potassium channel |
IC50 |
280000 |
nM |
= |
|
|
|
Homo sapiens |
Bioorg. Med. Chem. Lett., 2004, 14 (18), 4771-4777 |
ChEMBL |
1449647 |
data from ChEMBL25 data validity problem., unknown assay type |
| 21 | HG-0260086-0-00 |
hERG |
binding |
DRUGMATRIX: Potassium Channel HERG radioligand binding (ligand: [3H] Astemizole) |
Inhibition |
50 |
% |
< |
Not Active (inhibition < 50% @ 10 uM and thus dose-reponse curve not measured) |
10 |
HEK293 |
Homo sapiens |
DrugMatrix in vitro pharmacology data |
ChEMBL |
7741976 |
data from ChEMBL25 |
| 22 | HG-0260086-0-00 |
hERG |
patch clamp |
Inhibition of human ERG expressed in CHO cells by whole cell patch clamp technique |
IC50 |
50118.7 |
nM |
= |
|
|
CHO |
Homo sapiens |
Bioorg. Med. Chem., 2008, 16 (11), 6252-6260 |
ChEMBL |
2358291 |
data from ChEMBL25 Potential duplicate. |
| 23 | HG-0260086-0-00 |
hERG |
other |
Inhibition of hERG K channel |
IC50 |
275000 |
nM |
= |
|
|
|
Homo sapiens |
Cardiovasc. Res., 2011, 91 (), 53-61 |
ChEMBL |
15257964 |
data from ChEMBL25 data validity problem., unknown assay type |
| 24 | HG-0260086-0-00 |
hERG |
other |
Inhibition of human ERG |
IC50 |
50118.7 |
nM |
= |
|
|
|
Homo sapiens |
Eur. J. Med. Chem., 2011, 46 (2), 618-630 |
ChEMBL |
5218907 |
data from ChEMBL25, unknown assay type |
| 25 | HG-0260086-0-00 |
hERG |
patch clamp |
hERG-U2OS cellular assay by FluxORTM thallium flux assay. PubChem AID:588834 |
Potency |
|
|
|
Phenotype:Inactive, Activity outcome:Inactive |
|
U2OS |
|
http://assay.nih.gov/htsws/rest/display/herg-trans-p1/NCGC00024983-02/38505689 |
PubChem_CID |
588834 |
Data from PubChem AID588834 by NCGC. Curve_Description: Compound QC:QC'd by SigmaAldrich |
| 26 | HG-0260086-0-00 |
hERG |
patch clamp |
hERG-U2OS cellular assay by FluxORTM thallium flux assay. PubChem AID:588834 |
Potency |
|
|
|
Phenotype:Inactive, Activity outcome:Inactive |
|
U2OS |
|
http://assay.nih.gov/htsws/rest/display/herg-trans-p2/NCGC00024983-07/39155694 |
PubChem_CID |
588834 |
Data from PubChem AID588834 by NCGC. Curve_Description: Compound QC:QC'd by BIOMOL |
| 27 | HG-0260086-0-00 |
hERG |
patch clamp |
hERG-U2OS cellular assay by FluxORTM thallium flux assay. PubChem AID:588834 |
Potency |
|
|
|
Phenotype:Inactive, Activity outcome:Inactive |
|
U2OS |
|
http://assay.nih.gov/htsws/rest/display/herg-trans-ntp-p2/AB08082716-01/39869162 |
PubChem_CID |
588834 |
Data from PubChem AID588834 by NCGC. Curve_Description: Compound QC:QC'd by NIEHS/NTP |
| 28 | HG-0260086-0-00 |
hERG |
patch clamp |
hERG inhibition(%) at 10uM by Ionworks Quattro (MDC, Sunnyvale, CA) in population patch clamp (PPC) mode |
inhibition |
1.70172 |
% |
= |
|
10 |
CHO |
Homo sapiens |
PLoS One. 2015; 10(2): e0118324. |
hERG Central(PubChem_SID) |
|
Data from PLoS One. 2015; 10(2): e0118324 containing hERG Central data. hERG inhibition (%) at 1uM=6.5329% |
